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Image Search Results
Journal: The FASEB Journal
Article Title: Regionally selective cardiovascular responses to adenosine A 2A and A 2B receptor activation
doi: 10.1096/fj.202101945R
Figure Lengend Snippet: Glosensor cyclic AMP responses to CGS 21680 and BAY 60‐6583 in HEK293G cells. (A) Concentration‐response curves for agonist‐stimulated cAMP Glosensor luminescence responses in HEK293G cells in response to CGS 21680 and BAY 60‐6583. (B) The effect of simultaneous addition of 100 µM BAY 60‐6583 on the concentration‐response curve to CGS 21680, following 2 h pretreatment with PSB 603 (0.1 µM). Also shown is the effect of simultaneous addition of 10 µM SCH 58261 on the responses to CGS 21680 in the presence of PSB 603 (0.1 µM). (C, D) The effect of 100 µM BAY 60‐6583 on the response to 10 µM formoterol in the (C) absence and (D) presence of 0.1 µM PSB 603. (E) The effect of simultaneous addition of 10 µM SCH 58261 on the response to 10 µM formoterol. Data represent mean ± SEM of the peak luminescence response of 5 separate experiments, each carried out in triplicate. Data are expressed as a percentage of the peak luminescence response obtained with 100 µM NECA measured in the same experiment. ** p < .05 (unpaired t ‐test)
Article Snippet: A clonal HEK 293 cell line stably expressing the
Techniques: Concentration Assay